AnaSpec AS-72263 SensoLyte® SARS-CoV-2 Papain-like Protease (PLpro) Activity Assay Kit *Fluorimetric*，SensoLyte®SARS-CoV-2類木瓜蛋白酶（PLpro）活性測定試劑盒*熒光法*，1 kit
AnaSpec ( 美國 )
AnaSpec公司成立于1993年，是一家為*生命科學研究，提供綜合蛋白質組學和基因組學解決方案的lingxian供應商。
公司產品主要分三大部分：提供專業多肽，檢測試劑，抗體檢測試劑盒；適合基礎研究的高通量篩選和新藥物研發相關試劑；多肽合成，抗體生產，寡核苷酸的合成等定制服務。
我們北京諾博萊德科技有限公司作為AnaSpec公司的經銷商為客戶提供，正規進口，貨期穩定，折扣好的服務標準。
AnaSpec AS-72263 SensoLyte® SARS-CoV-2 Papain-like Protease (PLpro) Activity Assay Kit *Fluorimetric*，SensoLyte®SARS-CoV-2類木瓜蛋白酶（PLpro）活性測定試劑盒*熒光法*，1 kit，產品簡介：
產品品牌：AnaSpec
產品名稱：SensoLyte®SARS-CoV-2類木瓜蛋白酶（PLpro）活性測定試劑盒*熒光法*
英文名稱：SensoLyte® SARS-CoV-2 Papain-like Protease (PLpro) Activity Assay Kit *Fluorimetric*
產品貨號：AS-72263
產品規格：1 kit
AnaSpec AS-72263 SensoLyte® SARS-CoV-2 Papain-like Protease (PLpro) Activity Assay Kit *Fluorimetric*，SensoLyte®SARS-CoV-2類木瓜蛋白酶（PLpro）活性測定試劑盒*熒光法*，1 kit，產品說明：
SensoLyte®520 SARS-CoV-2類木瓜蛋白酶/去泛素酶活性檢測試劑盒采用熒光肽底物檢測去泛素化活性。當活性木瓜蛋白酶樣蛋白酶切割底物時，在激發/發射=490 nm/520 nm時，會導致亮綠色熒光增加。該檢測方法可檢測樣品中低至7.8 ng/mL的活性木瓜蛋白酶。該試劑盒可用于檢測純化酶制劑、生物樣品中的酶活性，也可用于化合物篩選。
SARS-Cov-2木瓜蛋白酶樣蛋白酶（PLpro）編碼為非結構蛋白3（nsp3），參與病毒多蛋白的切割。這種酶水解泛素鏈，泛素鏈在炎癥過程中起重要作用，并從蛋白質中去除干擾素刺激的基因15（ISG15）修飾以逆轉抗病毒反應。PLpro作為SARS-CoV-2復制和感染宿主過程中的一種必需酶，已成為冠狀病毒抑制劑開發的一個有吸引力的藥物靶點。
The SensoLyte® 520 SARS-CoV-2 Papain-like Protease/ Deubiquitinase Activity Assay Kit employs a fluorescent peptide substrate for the detection of deubiquitnation activity. When active Papain-like Protease cleaves the substrate, it results in an increase of bright green fluorescence at excitation/emission = 490 nm/520 nm. This assay can detect as low as 7.8 ng/mL active Papain-like Protease in the sample. This kit can be used to detect enzyme activity in purified enzyme preparations, biological samples and can also be applied for the compound screening.
SARS-Cov-2 Papain-like Protease (PLpro) is encoded as non-structural protein 3 (nsp3) and it is involved in the cleavage of the viral polyprotein. This enzyme hydrolyzes ubiquitin chains which are important in the inflammation process and also removes interferon-stimulated gene 15 (ISG15) modifications from proteins to reverse antiviral responses. As an essential enzyme in the process of SARS-CoV-2 replication and infection of the host, PLpro has been an attractive drug target for the coronavirus inhibitor development.
Kits components 產品組件：
Component A: Papain-like Protease Deubiquitination substrate Ex/Em=490 nm/520 nm upon cleavage; 400µM, 50µL Component B: Rh110 fluorescence reference standard, Ex/Em=490 nm/520 nm; 400µM, 15µL Component C: Recombinant SARS-CoV-2 Papain-like protease; 0.1mg/mL, 10µL Component D: 2X Assay Buffer, 25mL Component E: Inhibitor (GRL0617); 10µM, 10µL
組分A：木瓜蛋白酶類去泛素化底物Ex/Em=490nm/520nm；400µM，50µL組分B:Rh110熒光對照品，Ex/Em=490 nm/520 nm；400µM，15µL組分C：重組SARS-CoV-2木瓜蛋白酶；0.1mg/mL，10µL組分D:2X分析緩沖液，25mL組分E：抑制劑（GRL0617）；10µM，10µL
AnaSpec品牌部分產品貨號：
品牌 | 貨號 | 名稱 | 規格 |
AnaSpec | AS-72262 | SensoLyte® SARS-CoV-2 3CL Protease Activity Assay Kit *Fluorimetric*， | 1 kit |
AnaSpec | AS-72263 | SensoLyte® SARS-CoV-2 Papain-like Protease (PLpro) Activity Assay Kit | 1 kit |
AnaSpec | AS-55551 | SensoLyte® Anti-Human β-Amyloid (1-40) Quantitative ELISA Kit *Colorimetric* | 1 kit |
AnaSpec | AS-55552 | SensoLyte® Anti-Human β-Amyloid (1-42) Quantitative ELISA Kit *Colorimetric* | 1 kit |
AnaSpec | AS-72246 | AnaTag™ Europium Protein Labeling Kit | 1 kit |
AnaSpec | AS-72247 | AnaTag™ Protein Labeling Kit for TR-FRET - 1 kit | 1 kit |
AnaSpec | AS-71120 | SensoLyte® AMC Caspase Profiling Kit Fluorimetric - 1 kit | 1 kit |
AnaSpec | AS-71121 | SensoLyte® AMC Caspase Substrate Sampler Kit Fluorimetric - 1 kit | 1 kit |
AnaSpec | AS-71124 | SensoLyte® Green Protease Assay Kit Fluorimetric - 1 kit | 1 kit |
AnaSpec | AS-71126 | SensoLyte® 490 HCV Protease Assay Kit Fluorimetric 500 assays - 1 kit | 1 kit |
AnaSpec | AS-71127 | SensoLyte® 490 HIV Protease Assay Kit Fluorimetric - 1 kit | 1 kit |
AnaSpec | AS-71128 | SensoLyte® 490 MMP-1 Assay Kit Fluorimetric - 1 kit | 1 kit |
AnaSpec | AS-71129 | SensoLyte® 490 MMP-2 Assay Kit Fluorimetric - 1 kit | 1 kit |
AnaSpec | AS-72040 | SensoLyte® 520 Renin Assay Kit Fluorimetric - 1 kit | 1 kit |
AnaSpec | AS-72122 | SensoLyte® Luminescent Alkaline Phosphatase Assay Kit Luminometric - 1 kit | 1 kit |
AnaSpec | AS-72129 | SensoLyte® 520 Thrombin Activity Assay Kit Fluorimetric - 1 kit | 1 kit |
AnaSpec | AS-20276 | Beta-Amyloid (1-42) | 1 mg |
AnaSpec | AS-20276-25 | Beta-Amyloid (1-42) | 25 mg |
AnaSpec | AS-20276-5 | Beta-Amyloid (1-42) | 5 mg |
AnaSpec | AS-20291 | Threonine Phosphopeptide, PKC Substrate 4, phosphorylated - 1 mg | 1 mg |
AnaSpec | AS-20292 | Insulin Receptor (1142-1153), pTyr1146 - 1 mg | 1 mg |
AnaSpec | AS-21158 | HA peptide | 5 mg |
AnaSpec | AS-24467 | Exendin (9-39) | 0.5 mg |
AnaSpec | AS-24468 | Exendin (9-39) | 1 mg |
AnaSpec | AS-64129-05 | Beta-Amyloid (1-42) • HFIP | 0.5 mg |
AnaSpec | AS-64129-1 | Beta-Amyloid (1-42) • HFIP | 1 mg |
AnaSpec | AS-72215 | AggreSure ?-Amyloid (1-40), human - 0.25 mg | 0.25 mg NET peptide |
AnaSpec | AS-72216 | AggreSure ?-Amyloid (1-42), human - 0.25 mg | 0.25 mg NET peptide |
AnaSpec | AS-72049 | AnaTag™ HiLyte™ Fluor 647 Protein Labeling Kit - 1 kit | 1 kit |
AnaSpec | AS-72050 | AnaTag™ HiLyte™ Fluor 647 Microscale Protein Labeling Kit - 1 kit | 1 kit |
AnaSpec | AS-72111 | AnaTag™ APC Labeling Kit - 1 kit | 1 kit |
AnaSpec | AS-72113 | AnaTag™ R - PE Labeling Kit - 1 kit | 1 kit |
AnaSpec | AS-55158-100 | Recombinant human MOG (1-125) | 100 µg |
AnaSpec | AS-55158-1000 | Recombinant human MOG (1-125) | 1000 µg |
AnaSpec | AS-55158-500 | Recombinant human MOG (1-125) | 500 µg |
AnaSpec | AS-55525-1 | MMP-12 (Catalytic Domain), human recombinant | 1 µg |
AnaSpec | AS-55525-10 | MMP-12 (Catalytic Domain), human recombinant | 10 µg |
AnaSpec | AS-55525-50 | MMP-12 (Catalytic Domain), human recombinant | 50 µg |
AnaSpec | AS-72237 | Protein A-HiLyte™ Fluor 647 Conjugate - 1 mg | 1 mg |
AnaSpec | AS-72239 | Protein A-HiLyte™ Fluor 750 Conjugate - 1 mg | 1 mg |
AnaSpec | AS-72237 | Protein A-HiLyte™ Fluor 647 Conjugate - 1 mg | 1 mg |
AnaSpec | AS-72239 | Protein A-HiLyte™ Fluor 750 Conjugate - 1 mg | 1 mg |
如需其他產品請聯系本店客服。
